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KMID : 0363020050350020461
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.461 ~ p.474
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A Study of Safflower Seed Extracts on Bone Formation in Vitro
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이성진
최호철/선기종/송제봉/피성희/유형근/신형식
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Abstract
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The ultimate goal of periodontal therapy is the regeneration of periodontal tissue and the
repair of function. For more than a decade there have been many efforts to develop materials and methods of treatment to promote periodontal tissue regeneration. Recently many
efforts are concentrated on the regeneration potential of material used in traditional medicine.
Safflower(Carthamus tinctorius L.) seed extract(SSE) have long clinically used in Korea to
promote bone formation and prevent osteoporosis.
The purpose of this study was to examine the effects of SSE on bone formation in human
osteoblastic cell line. Human fetal osteoblastic cell line(hFOB 1.19) was cultured with DMEMand SSE(1㎍/㎖, 10㎍/㎖, 100㎍/㎖, 1mg/㎖) at 34℃ with 5% CO2 in 100% humidity. The
proliferation, differentiation of the cell was evaluated by several experiments.
Cell proliferation was significantly increased at 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE after 3
and 7 days incubation(p＜0.05). Cell spreading assay was significantly increased at 100㎍/㎖of SSE after 3 days and 1㎍/㎖, 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE after 7 days(p＜0.05).
Alkaline Phosphatase(ALP) level was significantly increased in 10㎍/㎖, 100㎍/㎖ of SSE(p
＜0.05). Collagen synthesis was significantly increased at 10㎍/㎖, 100㎍/㎖, 1mg/㎖ of SSE(p
＜0.05). A quantified calcium accumulation was significantly increased at 10㎍/㎖, 100㎍/㎖ of
SSE(p＜0.05). ALP and osteocalcin mRNA was expressed in 100㎍/㎖ of SSE by RT-PCR.
These results indicate that SSE are capable of increasing osteoblasts mineralization and
may play an important role in bone formation.
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KEYWORD
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Safflower Seed Extracts, hFOB 1.19, bone formation
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